GSM5208155: Piglet_control_vehicle_sample9; Sus scrofa; Bisulfite-Seq - SRA

SRX10434830: GSM5208155: Piglet_control_vehicle_sample9; Sus scrofa; Bisulfite-Seq
1 ILLUMINA (Illumina HiSeq 3000) run: 16.4M spots, 793.1M bases, 188.1Mb downloads

Submitted by: NCBI (GEO)

Study: Methylome analysis by reduced representation bisulfite sequencing (RRBS) from adipose tissue samples of piglets from a swine model of maternal overfeeding and/or restriction

PRJNA716956 • SRP311951 • All experiments • All runs

show Abstracthide Abstract

Maternal overfeeding and/or restriction may have effect on the metabolic and epigenetic programming of offspring's adipose tissue. The study tested the potential of postnatal metformin teatment to reverse these changes using a swine animal model. Overall design: We designed a swine model using pregnant sows distributed in 3 groups of diet intervention (control, overfed and restricted) through pregnancy. At birth, piglets underwent standard lactation without maternal diet intervention. Within each maternal group, piglets were rambdomly assigned to two different tratment interventions: metformin or vehicle. All piglets were sacrified at weaning and visceral adipose tissue samples were collected.

Sample: Piglet_control_vehicle_sample9

SAMN18472985 • SRS8568353 • All experiments • All runs

Organism: Sus scrofa

Library:

Instrument: Illumina HiSeq 3000

Strategy: Bisulfite-Seq

Source: GENOMIC

Selection: Reduced Representation

Layout: SINGLE

Construction protocol: High quality genomic DNA (400ng) was isolated from adipose tissue samples with Gentra PureGene tissue kit (Qiagen) according to manufacturer's instructions. DNA was bisulfited with the EZ DNA Methylation-Gold Kit (Zymo Research), according to manufacturer's instructions. Bisulfite conversion rate was over 99%. Libraries were were prepared for sequencing using standard Illumina protocols and Sequencing was performed on a HiSeq 3000 (Illumina).

Experiment attributes:

GEO Accession: GSM5208155

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 16.4M spots, 793.1M bases, 188.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR14060211	16,421,506	793.1M	188.1Mb	2021-12-17

ID:: 13830897

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity